| 產(chǎn)品描述： | PS-48 是 phosphoinositide-dependent protein kinase-1 (PDK1) 的變構(gòu)激活劑 |
| 靶點： |
PDK1;PDK |
| 體內(nèi)研究： |
In vivo, the PDK1 agonist PS48 can maintain the bone mass of mice treated with dexamethasone |
| 細胞實驗： |
Cell lines: MC3T3-E1 cells Concentrations: 5 μM Incubation Time: -- Method: MC3T3-E1 cells are cultured in an osteogenic differentiation medium containing 4 mM glycerophosphate and 25 μg/mL ascorbic acid until 70% confluency. Dexamethasone (final concentration of ethanol, 0.01%, vol/vol) at different concentrations is then added to the osteogenic differentiation medium for 14 days. The culture medium is replaced every two days. MC3T3-E1 cells are cultured in a culture medium containing 10?7 M dexamethasone supplemented with or without PS48 (5 μM). |
| 動物實驗： |
Animal Models: 8-week-old male C57BL/6J mice Dosages: -- Administration: -- |
| 參考文獻： |
1. Valerie Hindie, et al.Nat Chem Biol. 2009 Oct;5(10):758-64. 2. Wen-Ning Xu, et al. Front Endocrinol (Lausanne). 2020 Jan 28;10:922. |
| 溶解性： |
Soluble in DMSO、Ethanol |
| 保存條件： |
-20℃ |
| 配置溶液濃度參考： |
|
1mg |
5mg |
10mg |
| 1 mM |
3.487 ml |
17.437 ml |
34.873 ml |
| 5 mM |
0.697 ml |
3.487 ml |
6.975 ml |
| 10 mM |
0.349 ml |
1.744 ml |
3.487 ml |
| 50 mM |
0.07 ml |
0.349 ml |
0.697 ml |
|
| 注意： |
部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。 |
上海工商
危險品化學(xué)品經(jīng)營許可證（不帶存儲）
營業(yè)執(zhí)照(三證合一)
北京